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Double-Blind,
Placebo-Controlled Study of the Effects of LifePak® Supplementation
on Antioxidant Status, Free Radical Activity and Resistance to LDL-Oxidation
C.R.
Smidt, Ph.D.,1 D.L. Woehler, R.D.,2 R.J. Seidehamel, Ph.D.,3 S. Devaraj,
Ph.D.,4 I. Jialal, M.D., Ph.D.4 ABSTRACT
Objective:
The present study investigated whether LifePak®, a comprehensive
optimum potency multiple nutrient supplement, can improve antioxidant
status, decrease free radical activity, and improve resistance to LDL-oxidation
in healthy volunteers. Design:
One-hundred-fifty non-smokers, ages 18-65 years, were recruited in the
Houston, Texas area. Subjects were not taking any other dietary supplements
and consumed typical American diets with less than 5 servings of fruits
and vegetables. Seventy-five subjects were supplemented with LifePak®
and another 75 subjects received placebo (each in 2 packets of 3 capsules
each daily) for 6 weeks. Measurements:
Blood and urine samples were taken at the start and end of the supplementation
period and analyzed for serum antioxidant nutrients, such as vitamin
C, vitamin E, carotenoids and vitamin A, as well as serum oxygen radical
absorption capacity, and markers of free radical damage, including serum
total alkenals, hydroperoxides, and urinary alkenals, 8- hydroxyguanosine,
and 8-epi-PGF 2 . Plasma samples were prepared for LDL (low-density
lipoprotein) oxidizability assays. Results:
LifePak® supplementation significantly improved antioxidant status
as evidenced by increased serum levels of -carotene (from 0.236+0.200
µg/ml to 0.461+0.245 µg/ml; p<0.001), -carotene (from
62+54 ng/ml to 355+194 ng/ml; p<0.001), and vitamin E ( -tocopherol,
from 8.94+2.06 µg/ml to 15.96+5.21 µg/ml, p<0.001), with
no changes in the placebo group. LifePak® significantly increased
total serum oxygen radical absorption capacity by 5.0% (p<0.05) and
lowered serum total alkenals by 9.6% (p<0.001). LifePak® effectively
improved LDL's resistance to oxidation, as the lag time was prolonged
by about 18% (from 46.0+10.2 min to 54.5+13.9 min; p<0.001). Conclusions: LifePak® supplementation for 6 weeks significantly increased antioxidant status, reduced serum alkenals, and improved LDL resistance to oxidation in healthy non-smokers consuming typical U.S. diets. 1 Interior
Design Nutritionals, Nu Skin Enterprises, Provo, UT
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